ABSTRACT
OBJECTIVE@#To investigate the effect of podophyllotoxin on human gastric cancer cell line SGC 7901 cells in vitro and in vivo, and to provide evidence for treatment of gastric cancer with podophyllotoxin.@*METHODS@#SGC 7901 cells were treated with podophyllotoxin at various concentrations for different time. MTT assay, colony formation assay, and flow cytometry were performed to determine the effect of podophyllotoxin on the growth and apoptosis of SGC 7901 cells in vitro. Furthermore, xenograft experiment was performed to detect the effect of podophyllotoxin on the in vivo tumorigenicity of SGC 7901 cells.@*RESULTS@#Podophyllotoxin inhibited the growth of SGC 7901 cells in a concentration- and time-dependent manner, decreased the colony formation rate of the cells, induced SGC 7901 cell apoptosis, arrested SGC 7901 cells at G2/M phases of cell cycle, and decreased the volume and weight of SGC 7901 cell xenografts in nude mice.@*CONCLUSION@#Podophyllotoxin inhibits the growth of human gastric cancer SGC 7901 cells, induce the apoptosis of SGC 7901 cells in vitro, and inhibit the in vivo tumorigenicity of SGC 7901 cells, which provides evidence for treatment of gastric cancer using podophyllotoxin.
Subject(s)
Animals , Female , Humans , Mice , Antineoplastic Agents, Phytogenic , Pharmacology , Apoptosis , Mice, Inbred BALB C , Mice, Nude , Neoplasm Transplantation , Podophyllotoxin , Pharmacology , Stomach Neoplasms , Pathology , Tumor Cells, CulturedABSTRACT
<p><b>OBJECTIVE</b>To study the optimum technology of extracting bioactive component named geniposidic acid in Eucommia Ulmiodes by orthogonal test.</p><p><b>METHOD</b>An orthogonal test table L16(4(5)) was used with the factors of extracting time, temperature, times, ethanol consistency and the ratio of solid to liquid. The content of geniposideic acid was determined by RP-HPLC.</p><p><b>RESULT</b>The optimum technology was: temperature 50 degrees C, time 1 h, once, consistency 80%, and the ratio of solid and liquid 1:12. Under the condition, the best material of Eucommia Ulmiodes for extracting geniposidic acid was chosen.</p><p><b>CONCLUSION</b>The technology can provide some theoretic advice for industry.</p>
Subject(s)
Chromatography, High Pressure Liquid , Ethanol , Eucommiaceae , Chemistry , Glucosides , Iridoid Glucosides , Iridoids , Plant Bark , Chemistry , Plants, Medicinal , Chemistry , Technology, Pharmaceutical , Methods , Temperature , TimeABSTRACT
<p><b>OBJECTIVE</b>To supply basis for the establishment of quality standard of Cryptolepis buchanaii.</p><p><b>METHOD</b>Characters of crude drugs, microscopic characteristic as well as UV spectrum of the herb were studied.</p><p><b>RESULT</b>Laticifers were found in the cortex and pith of the stem; much papillary non-glandular hair was found covering the stomata in the sub-cuticle of the leaf.</p><p><b>CONCLUSION</b>The results can be employed as the basis for identifying the herb.</p>